تأثیر دوازده هفته تمرینات ترکیبی بر تنظیم نشانگرهای التهابی زنان یائسه مستعد سرطان سینه (مقاله علمی وزارت علوم)
درجه علمی: نشریه علمی (وزارت علوم)
آرشیو
چکیده
مقدمه: هدف از پژوهش حاضر تعیین اثر تمرین ترکیبی بر بیان ژن برخی از سایتوکاین های پیش التهابی و ارزیابی سطوح هورمونی در زنان یائسه چاق است. روش پژوهش: در این تحقیق نیمه تجربی 30 زن یائسه کم تحرک و چاق انتخاب شده و به طور تصادفی به دو گروه تجربی (15 نفر) و گروه کنترل (15 نفر) تقسیم شدند. گروه تجربی به مدت 12 هفته، پنج روز در هفته، شامل دو جلسه تمرین مقاومتی به مدت 60 دقیقه و 3 جلسه تمرین هوازی به مدت 30 دقیقه، شرکت کردند. پیش و پس از مداخله، نمونه خون برای ارزیابی استرادیول، استرادیول آزاد و میزان بیان mRNA ژن های اینترلوکین-6 (IL-6) و تومور نکروزدهنده آلفا (TNF-α) از داوطلبان اخذ شد. بیان کمی ژن با استفاده از روش 2 -∆∆ct و از طریق نرم افزار REST ارزیابی شد. از آزمون شاپیروویلک برای بررسی توزیع طبیعی داده ها و از تحلیل واریانس در اندازه گیری های مکرر برای مقایسه گروه ها در سطح معناداری α<0.05 استفاده شد. یافته ها: در پاسخ به پروتکل تمرینی، کاهش در توده چربی (6/9- درصد) و افزایش توده بدون چربی (2/4+ درصد) در گروه تجربی مشاهده شد. سطوح استرادیول آزاد (10/70- درصد ) و میزان بیان ژن IL-6 ( P=0.003) و TNF-α (P=0.000) کاهش معناداری در گروه تجربی نسبت به گروه کنترل داشت. اما تفاوت معناداری در استرادیول برای گروه تجربی وجود نداشت (P<0.05). نتیجه گیری: به نظر می رسد تمرین ترکیبی و کاهش توده چربی ناشی از فعالیت، پروفایل هورمون های جنسی و میزان بیان ژن IL-6 و TNF-α را در زنان یائسه مستعد BC تغییر می دهد. بنابراین این تحقیق شواهد بیشتری از تعامل پیچیده بین نشانگرهای التهابی، بافت چربی و توده عضلانی را در این گروه از زنان ارائه می کند.The Effect of Twelve-Week Concurrent Training on the Regulation of Inflammatory Markers in Postmenopausal Women Prone to Breast Cancer
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods : In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2 -∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion : The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods : In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2 -∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion : The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.
Introduction: The present study aimed to determine the effect of 12-week concurrent training on gene expression of some pro-inflammatory cytokines and to evaluate hormone levels of obese postmenopausal women.
Methods : In this quasi-experimental research, 30 sedentary and obese PW were recruited and randomly divided into Concurrent Training (CT, n=15) and Control (CG, n=15) groups. Participants of the experimental group took part in 12-week supervised intervention training for five days a week, including two sessions of 60-minute resistance training and three sessions of 30-minute aerobic training. Blood samples were taken from the volunteers to measure estradiol, free estradiol, and mRNA expression levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α). Quantitative gene expression was evaluated using the 2 -∆∆ct method and REST software. The Shapiro-Wilk test was used to check the normal distribution of the data, and repeated measurements analysis of variance (ANOVA) tests were used to compare the groups at a significance level of α=0.05.
Results: In response to the training protocol, the CT group experienced a reduction in fat mass (-9.6%; P<0.05) and an increase in lean body mass (2.4%; P<0.05). The Circulating levels of free Estradiol (−10.70%; P<0.05), and levels of gene expression of IL-6 (P=0.03) and TNF-α (P=0.000) significantly decreased in the CT group compared with the CG group. But there was no significant difference in Estradiol in the CT group compared with the CG group (P<0.05).
Conclusion : The concurrent training and exercise-induced fat mass loss seem to modify the sex hormones profile and levels of gene expression of IL-6 and TNF-α in PW Prone to breast cancer. Thus, this study provides additional evidence of the intricate interaction of inflammatory markers, adipose tissue, and muscle mass in PW Prone to breast cancer.