Stress is one of the influential factors in people's performance. Job and existing conditions of working environment can cause stress. One of the jobs that due to its nature is causing too much stress is nursing job. Various factors are involved in reducing stress, of which are spiritual skills. This study was conducted to evaluate the effect of training spiritual skills on stress and stressful features of nursing mothers. The study population of this semi-experimental with pre-test and post-test research design with control group, consisted of all female nurses of Milad Hospital (150 people) whom complete the questionnaire. 30 individuals with higher scores in stress were randomly assigned to experimental and control groups. Data collection tools include spiritual skill training package and Abidin parental stress index (PSI) with parental realm (54 items). Data were analyzed using analysis of covariance method & Levin univariate ANOVA. The hypothesis that training spiritual skills is effective in reducing nursing mothers' job stress is confirmed (F=24.977; Df=1 and 27; Sig=0.001). The results of the study imply that spiritual skill training was influential in reducing stress and stressful features of nursing mothers