The study aimed to examine the gender-adjusted impact of personality factors on the motivations of e-sports participants. Descriptive research was conducted using structural equation modeling. The sample consisted of 763 individuals (599 men and 164 women) selected through simple random sampling, with statistical power set at 80% and a significance level of 0.01. The research variables, including participation structure and personality traits, were measured using the NEO Five-Factor Inventory (NEO-FFI) short-form questionnaire (Gassling et al., 2003) and the Motivational Participation Questionnaire` of Cianfrone et al. (2011). The findings supported all research hypotheses regarding the personality traits and motivations of e-sports players, even after adjusting for gender. The results indicated that brunerism, consensus, duty, psychology, and acceptance of experience were significant motivators for e-sports players, and the gender-adjusted analysis confirmed the research hypotheses in both male and female groups. Therefore, considering the personalities of e-sports players in sports marketing can enhance their engagement, as their motivations to participate are influenced by their traits.