Background : Low back pain (LBP) can cause changes in muscle activity in affected individuals, making it difficult for them to contract their muscles effectively and engage in physical activities. Manual chiropractic techniques have been proposed as a potential solution to improve muscle orientation and function in individuals with LBP. Aim: The purpose of this study was to investigate the effect of manual techniques on maximum muscle activity electromyography, functional disability, and pain in women with non-specific chronic low back pain. Materials and Methods: Twenty women between the ages of 30 and 50, who had been experiencing NSCLBP for over three months with no history of spinal surgery or postural abnormalities, and were able to perform daily activities after providing consent, were recruited from a sports medicine clinic to participate in the study. In the pre-test, the Oswestry Disability Index (ODI) was used to assess functional disability, and the Visual Analogue Scale (VAS) was used to assess pain levels. Additionally, the maximum muscle activity of the multifidus and lumbar quadratus lumborum (QL) was measured using EMG. Chiropractic manipulation techniques were then implemented in the experimental group, with three sessions per week for six weeks, within the clinic. The same tests were repeated in the post-test. The ANOVA test was used to compare the chiropractic and control groups, with a significance level of α ≤ 0.05. Results : The study results indicate that chiropractic manual techniques were effective in reducing lumbar EMG activity ( P = 0.0001), reducing pain index ( P = 0.002), and improving functional disability ( P = 0.001) significantly. Conclusion : The study findings suggest that six weeks of chiropractic manipulation techniques can induce a feeling of relaxation, reduce pain, and passively strengthen the lumbar muscles. These improvements in muscle condition and functional disability may lead to a reduction in pain experienced by individuals with NSCLBP.