If culture is seen as a framework of "should" and "should not," defensive culture is based on issues, which are available in the society. Issues that differ from one society to another and are influenced by the strategic culture of the countries. So, the present study aimed to answer the question that what are the most important components of a defensive culture in the framework of the strategic culture of Iran, based on the Supreme Leader's views. Based on the results, the most important components influenced by Iran's strategic. culture include martyrdom and Ashura culture, deepening insight and recognizing enemy, Islamic-Iranian identity and national unity, self-confidence and belief in divine victory, denial of oppression and defense of the oppressed, defensive diplomacy, democratic defense, and multilateral defense which can be effective in adopting an appropriate defensive strategy and enhancing the defensive capability of the Islamic Republic of Iran against enemies. In this article an analytical-descriptive method is used.