Pinpointing the role of language, emotion, and culture in intercultural interactions as the three basic needs of intercultural interactions is one of the most important problems in the intercultural field. Therefore, this paper probed the role of language, emotion, and culture simultaneously in intercultural communication in Iran. By using convenience sampling, one hundred Iranian language teachers were chosen and their language proficiency and ICC levels were specified by using Oxford Placement Test (OPT) and intercultural communicative competence (ICC) questionnaire. Then, the researchers used exploratory factor analysis (EFA) to classify ICC questions under language, emotion, and culture categories. After classifying the questions, the researchers used confirmatory factor analysis (CFA), standard solution coefficient, standard path coefficient, T value, and Sobel formula to analyze the collected data from the ICC questionnaire based on participants' answers. The results revealed that language, culture, and emotion were the main macro-elements of intercultural communication. Also, it was shown that language was the most important need and macro-element in intercultural communication and then the second and third place went to culture and emotion respectively. It was found that emotion acted as a mediator variable between language and culture and there was a relationship among language, culture, and emotion, too. This study was of high importance for language teachers, curriculum designers, textbook writers, intercultural experts, and cultural psychologists