Objective: The aim of the study was to investigate the effect of organizational support on life satisfaction and job satisfaction of female coaches in Isfahan with the mediating role of work-family conflict. Methods: It was a descriptive – field study and a practical one. The research population was female coaches in Isfahan and 206 coaches in sport complexes were selected through convenience sampling. Cammann’s questionnaire (1983) was used to evaluate job satisfaction, and Diener et al.'s (1985) questionnaire was used to measure life satisfaction. Netemeyer et al.’s questionnaire (1996) was used to assess work-family conflict, and organizational support scale of Eizenberger et al. (1996) was used for measuring organizational support. The reliability of questionnaire was confirmed by Cronbach’s alpha; and content, face and structural validity were also conducted to investigate the questionnaire validity. To measure the validity and fitness of the model, structural equation modeling by AMOS20 has been applied. Results: The findings demonstrated that “Organizational support” had a significant effect on “work-family conflict” (β= -0.37), “Job satisfaction” (β= 0.54), “Life satisfaction” (β= 0.48). In addition, “work-family conflict” had a significant effect on “Job satisfaction” (β= -0.31), and “Life satisfaction” (β= -0.26). Moreover, the Sobel test confirmed the role of the mediating variable. The fit indices also confirmed the research model fitness. Conclusion: According to the role of organizational support in decreasing the work-family conflict as well as the effect of work-family conflict on job satisfaction and life satisfaction, to pave the way for presence of females in sport field, sports organizations should plan their strategies by focusing on the concepts and results of this research.