As in many other countries, universities in Indonesia have been closed since March 2020 due to the COVID-19 pandemic lockdown, and lecturers have been forced to adapt to online learning. Students in this online course admitted to feeling anxious when studying public speaking. This descriptive quantitative study aims to analyze the potential factors (English oral proficiency, personality, lecturer's scoring criteria, classmates' responses, pronunciation, audience familiarity, unfamiliar speech topic, preparedness, English grammar, vocabulary) affecting the students' public speaking anxiety at four different stages. The respondents of this study were 60 undergraduate students. A questionnaire adapted from McCroskey (1970) was used, and data were analyzed using descriptive statistics of IBM SPSS statistics 26 software. The findings of this study revealed that the factors affecting the students' public speaking anxiety through online learning varied by stages. Through online learning, English oral proficiency and topic familiarity could be the most influential factors on students' public speaking anxiety. The findings of this study are useful for educators in helping EFL students overcome their public speaking anxiety and enabling them to perform better in public speaking.