The pivotal role of listening comprehension in second/foreign language learning requires that researchers conduct studies which investigate factors that affect test takers’ performances. The present study was set out to examine whether item modality (i.e., written vs. oral items) affects listening comprehension test performance. In addition, it investigated whether allowing test takers to take notes while listening would also affect their performances. To this end, two different tests, each containing 20 multiple choice items, were administered to 66 (35 female and 31 male) upper-intermediate EFL learners. The first test was administered to look into the role of item modality, and the second test was employed to investigate the effect of note-taking. The application of independent samples t-tests to analyze the data revealed that that test takers performed better when the items were provided in written rather than oral form, and that test takers’ performances did not differ significantly when they were allowed to take notes. More detailed findings and implications are discussed in the paper.