This study investigates the effects of gender, teaching experience, and brain dominance on English teachers’ teaching styles. In doing so, 68 English teachers (males and females) were selected from several language institutes in Shiraz, Iran. Considering the degree of teaching experience, the participants were categorized into three groups, namely novices, the moderately experienced, and professionals. Two instruments, including Hemispheric Dominance Test and Teaching Style Questionnaire were used to collect data. Using statistical package for the social science (SPSS) 20, the collected data were analyzed. The results of independent samples t-test revealed that male and female teachers differed significantly in terms of teaching styles, as far as the sub-component “formality” was concerned. Furthermore, the results of multivariate analysis of variance (MANOVA) test indicated that teaching styles in the three groups of teachers significantly differed. However, the analysis of MANOVA regarding the difference between brain dominance and teaching styles did not reveal any significant difference. Some related issues were also discussed.