Nowadays, teachers are expected to demonstrate a notable level of digital competence. Therefore, the current study investigated Iranian male and female pre-service and in-service EFL teachers’ digital competence. The study employed a mixed methods approach and three instruments including a questionnaire, structured interviews, and observations were utilized. Therefore, 157 Iranian in-service EFL teachers completed the DIGIGLO questionnaire. Also, 15 in-service and 15 pre-service EFL teachers participated in structured interviews. Moreover, 30 classes were observed. To analyze the qualitative data, thematic analysis was used and in the phase of quantitative analysis, descriptive statistics and independent sample t-tests were used. The results indicated that the level of Iranian in-service EFL teachers’ digital competence was moderate to high. In addition, some similarities and differences could be seen in the interview questions between in-service and pre-service EFL teachers. Moreover, it was revealed that there were no significant differences between male and female in-service EFL teachers’ digital competence. Furthermore, it was found that a majority of teachers incorporated digital technologies into their teaching practice. The results of this study can help curriculum developers and teacher educators realize a better conception of Iranian pre-service and in-service EFL teachers’ digital competence and their practices of using digital tools.