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Introduction: Nowadays, the use of artificial turf fields has become widespread. Given the high prevalence of noncontact injuries among female soccer players and high contribution of the Anterior Cruciate Ligament (ACL) injury, this research was conducted to compare biomechanical risk factors in the incidence of ACL injury for elite female soccer players during shearing maneuver and header on the natural grass and artificial turf fields. Methods: Sixteen players were selected from U-14 women’s national team by the convenience sampling method and started to perform the skills. Knee joint information was calculated by a 3D imaging system and a force plate. Statistical differences were reported using the dependent t-test at a significant level of 0.05. Results: The findings showed that the biomechanical behavior of the lower extremity while performing soccer skills on the natural grass was affected differently from that on the artificial turf. Results also demonstrated that the torque applied to the knee joint on the natural grass was higher than that on the artificial turf (P=0.039). Angular velocity on the artificial turf was also higher than that on the natural grass (P=0.006). Conclusion: The higher knee joint torque and the lower angular velocity on natural grass may result in a higher risk of ACL injury on the natural grass than on the artificial turf. Therefore, the use of prevention programs for ACL injury is crucial to minimize the incidence of lower extremity injuries.