The aim of the current study was to investigate whether Podcasts would have an effect on Iranian autonomous and non-autonomous EFL learners’ listening comprehension ability at pre-intermediate level. For this purpose, 60 Iranian pre-intermediate EFL learners at some Iranian institutes participated in the experiment of the study. Then they were divided into two groups based on an OPT and an autonomy questionnaire. The participants of each group were assigned randomly into two experimental groups (N=15) and two control groups (N=15). A pre-test was administered to the four groups of the study and then, the experimental groups received 5 sessions of teaching podcast. After the treatment, a posttest of L2 listening comprehension was administered to all groups of the study and finally the data were analyzed via a series of paired sample t-tests to see whether there is progress between the pretest and posttest scores of the groups, and a Two-way ANOVA was used to see the differences between posttests. The results revealed that Iranian autonomous and non-autonomous EFL learners’ listening comprehension ability got better after the treatment.