This study aims at investigating the interface between emotion and intonation patterns (more specifically, duration and pitch amplitude of speech). To this end, the acoustic properties of spectral parameters related to speech prosody are investigated. The results of acoustic and Statistical analysis show that mean level and range of FO in the contours vary strongly as a function of the degree of activation of the portrayed emotions. The results also indicate that FO/pitch and other vocal aspects affected by emotion and/or emotional arousal in collected samples of speech in Persian.