Mobile-Assisted Language Learning (MALL) is prevalent in English instruction to enhance students' learning interest and motivation. However, its effectiveness and practicality have rarely been well confirmed. The present study intended to investigate the effectiveness and practicality of utilizing a developed Mobile Learning Application (MLA) in listening instruction. A quasi-experimental design was applied in the study with a test and a questionnaire as research instruments. The pre-test and post-test were administered to examine the effects of administering MLA on 86 undergraduate students’ listening comprehension, and the questionnaire was employed to investigate 184 students’ perceptions of the practicality of MLA in mastering listening comprehension as data triangulation. Descriptive and inferential statistics were utilized to analyze the collected data. The results showed that a teacher-developed MLA is feasible for effective learning media to enhance students’ listening comprehension with the significant difference between pre-test and post-test scores. Moreover, it also accentuated that students' perception obtained a positive perception of the practicality of a developed MLA in mastering their listening comprehension. This finding encourages EFL teachers to use a mobile learning application in listening instruction to promote students’ independence in mastering listening skills.
