In recent years, there has been a growing recognition of learner autonomy; however, the roles of teachers and language materials in fostering in have not received due attention. This mixed methods study investigated the association between what Iranian teachers perceived of LA and what they thought about the role of textbooks in fostering EFL learners’ LA, and to what extent they believed the principles of LA in their classrooms. The participating teachers were 200 male and female teachers that taught Iranian tenth-grade EFL textbook in senior high schools of Qom, Arak, and Karaj. The first instrument was a questionnaire developed by Borg and Al-Busaidi (2012), which primarily focused on the teachers’ perspectives and opinions about LA. The second questionnaire was used to investigate how Iranian EFL teachers think the EFL textbook Vision 1 helps foster LA. The questionnaire was developed based on the LA principles (Fenner & Newby, 2000). The last instrument was an interview protocol that was developed by the researcher to gain a deeper understanding of teachers’ promoting LA in their teaching. The interview protocol also was based on Fenner and Newbys’ (2000) principles. For this study, the questionnaire data were collected through two questionnaires. Moreover, an interview protocol was used to gather qualitative data from 12 teachers, who participated in the interviews (i.e., 2 face-to-face and 10 online interviews). The result of the bivariate correlation test showed that there was a significant relationship between teachers’ perception of learner autonomy and their opinions about the LA-fostering role of the Iranian 10th-grade EFL textbook. The results of the interview analysis showed that the principles of learner autonomy were largely approved of by the interviewees. The study has some implications for textbook developers, teachers trainers, and teachers.